Coding

Part:BBa_K4041016:Design

Designed by: Derek Zhang   Group: iGEM21_HKIS   (2021-10-21)


Expression Cassette for pET-Yafl-Ulp1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1189
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 3445
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1147
    Illegal BglII site found at 1670
    Illegal BamHI site found at 2313
    Illegal BamHI site found at 2463
    Illegal BamHI site found at 2475
    Illegal BamHI site found at 2493
    Illegal BamHI site found at 2499
    Illegal XhoI site found at 3454
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1189
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1189
    Illegal AgeI site found at 2143
    Illegal AgeI site found at 2260
    Illegal AgeI site found at 3328
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No


Source

Cloning

References